Here we investigate these features in extract peptone dextrose (YPD) medium using a pair of monoclonal antibodies which were validated by Western blot, and LC MS/MS. CaEno abundantly presented in the cytosol distributing in a ring-shaped pattern along the cell wall;Surface display of CaEno was confirmed by whole cell ELISA and flow cytometry. Further laser confocal microscopy analysis revealed that the presence of the surface-located CaEno maintained stable during the culture.DAS-ELISA indicated that CaEno in the supernatant was gradually increased during culture and reached a maximum at 48 h and then the concentration remained stable until 120 h.