We investigated the spectroscopic properties of photosystem I from five different plant species (Arabidopsis thaliana, Spinacia oleracea, Zea mays, Spathiphyllum wallisii, Calathea roseopicta). In this dataset we present the absorption spectra, steady state fluorescence spectra at different temperatures (room temperature, 280-298 K, 77 K) and time resolved fluorescence data of the different PSI complexes. We also include the 77 K fluorescence spectra of the thylakoids and the DNA sequences of the lhca proteins that we extracted from the Arabidopsis Information Resource (TAIR).