IntroductionData underlying the publication: Depth-dependent scaling of axial distances in light microscopyPublication DOI: https://doi.org/10.1364/OPTICA.520595Repository created by Daan Boltje
AbstractIn volume fluorescence microscopy, refractive index matching is essential to minimize aberrations. There are however, common imaging scenarios, where a refractive index mismatch (RIM) between immersion and sample medium cannot be avoided. This RIM leads to an axial deformation in the acquired image data. Over the years, different axial scaling factors have been proposed to correct for this deformation. While some reports have suggested a depth-dependent axial deformation, so far none of the scaling theories has accounted for a depth-dependent, non-linear scaling. Here, we derive an analytical theory based on determining the leading constructive interference band in the objective lens pupil under RIM. We then use this to calculate a depth-dependent re-scaling factor as a function of the numerical aperture (NA), the refractive indices 𝑛₁ and 𝑛₂, and the wavelength 𝜆. We …