This dataset presents the data of our research about the effect of sample freezing on oxidative stress biomarkers (thiobarbituric acid reactive substances, protein carbonyl derivatives, total antioxidant capacity, superoxide dismutase and catalase activity) in different tissues (heart, skeletal muscles, brain) of mice in response to a maximum exercise. The markers were quantified in fresh and frozen tissues (1 to 6 months) in control and exercised animals.